ABSTRACT
Objective To investigate the effects of citric acid on the related indexes of salivary secretion under acid loading in or‐der to optimize the citric acid load method .Methods The saliva samples were collected from 10 young healthy volunteers at 1-90 s before ,at 91-120 s during and at 121-210 s after citric acid loading .The indexes were detected in saliva with mixed loading and after loading .The salivary alpha‐amylase(sAA ) activity ,pH value ,saliva flow rate ,total protein concentration in various groups were detected .The ratio values before and after the acid loading were compared among the groups .Results (1)The sAA activity , saliva pH value and total protein concentrations after acid loading were significantly increased compared before loading (P<0 .05) , moreover the ratio of after loading and before loading was greater than 1(P<0 .05);(2) however in the citric acid mixing ,the sAA activity ,saliva pH value and total protein concentration were decreased compared with before acid loading ,its ratio was less than 1 (P<0 .05) .Conclusion Citric acid affects the secretion result of acid loading saliva secretion ,it is suggested that the saliva under acid loading is separated treated and analyzed .
ABSTRACT
It is considered that olfactory stimulation by fragrance inhalation is one of the methods of relaxation. We examined the possibility by using fragrance inhalation essential oils, such as sweet orange oil and peppermint oil. We measured salivary alpha-amylase activity and state anxiety levels in undergraduate students before and after inhalation. Salivary alpha-amylase activity levels have been utilized to assess the sympathetic nervous activity.<br> Our results indicate that both salivary alpha-amylase activity and state anxiety levels are significantly reduced after fragrance inhalation of the sweet orange oil. Consequently, our research suggests that sweet orange essential oil has a relaxation effect.<br>